6625 Top Gun Street, Suite 103 San Diego, CA 92121. Alexa Fluor 647 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. ATTO647 NHS ester was purchased from Sigma-Aldrich (St. Louis, MO). This chapter lists the CAS registry number, chemical structure, chemical abstract (CA) index name, other chemical names, chemical/dye class, synthesis procedure, molecular formula, and molecular weight of Alexa Fluor 488 carboxylic acid succinimidyl ester. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Alexa Fluor Hg‐Link dye is a new generation of fluorescent probes that exhibit some advantages over other fluorescent dyes, such as being highly fluorescent over a broad pH range (pH 4 to 10), good water solubility, and higher photostability, allowing more time for image capture. Fisher Scientific - Arendalsvägen 16 - 418 78 Göteborg - Sweden © Thermo Fisher Scientific Inc. 입금계좌 : 신한은행 140-004-396660 (써모피셔 사이언티픽 솔루션스 유한회사), Get upto 200X more sensitivity in ICC/IHC/FISH for more clarity and definition with new. 50 µl (1 mg/ml) mouse anti-pip 2 or pip 3 (Echelon Inc) IgG monoclonal antibody were conjugated according to the manufacturer's instructions, with Alexa Fluor 647 carboxylic acid succinimidyl ester and Alexa Fluor 488 carboxylic acid succinimidyl ester (both Invitrogen), at a ratio of around 1–3 dye molecules per molecule of IgG. Alexa Fluor 647 is used for protein and antibody labeling, or nucleic acid applications with high labeling density. The chapter also includes references on the use and properties of Alexa Fluor 555 carboxylic acid succinimidyl ester. Reactivity: Aldehyde, carboxylic acid, ketone. face Alexa Fluor 647 was determined to be 99% by HPLC analysis. Reactivity: Primary amines. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. 대표번호 고객 헬프 데스크 : 1661-9555 (평일 09:00~18:00) Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 followed by CF633 or Alexa Fluor 647 goat anti-rabbit IgG conjugates. It presents a list of imaging/labeling applications and biological/medical applications of Alexa Fluor 555 carboxylic acid succinimidyl ester. Would you like to inquire about bulk pricing? 1 Product Result | Match Criteria: Product Name, Property, Description Handbook of Fluorescent Dyes and Probes. Fisher Scientific - Arendalsvägen 16 - 418 78 Göteborg - Sweden © Thermo Fisher Scientific Inc. This class includes far-red fluorescence-emitting dyes with excitation/emission maximum 646/662 nm. The secondary antibodies used were goat anti-guinea pig Alexa Fluor 546, goat anti-rabbit Alexa Fluor 488, and goat anti-mouse Alexa Fluor 647. Cells were imaged using a mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. For Research Use Only. NHS esters) through a stable amide bond. All Rights Reserved. Relative photostability of CF633 and Alexa Fluor 647 (AF647) goat anti-mouse conjugates. The dye has 4 sulfonate groups make it   high water soluble and less aggregation in the aqueous solution. Blood vessels were visualized by injecting 25 μg of DyLight 488–conjugated tomato lectin (green). Alexa Fluor 647 Carboxylic Acid, tris(triethylammonium) salt Compare Products: Select up to 4 products. Alexa Fluor® 647 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Cyanine 5 / Alexa Fluor 647 dyes are popular fluorescent dyes compatible with various detection techniques and standard filters. Images were obtained with a confocal microscope. Reproduction of any materials from the site is strictly forbidden without permission. Alexa Fluor® 488 Carboxylic Acid is a non-reactive form of Alexa Fluor® 488 that can be used as a reference standard for dye-conjugates. 주소 : 서울시 강남구 광평로 281 수서 오피스빌딩 12층(06349) Alexa Fluor® 647 is a bright, far red dye with excitation ideally suited to the 633 nm laser line. Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 Conjugate. Alexa Fluor® 488 NHS Ester AFDye 488 Acid. Anti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor 647 Conjugate 1 Product Result | Match Criteria: Product Name, Property, Description The dye is water soluble and pH-insensitive from pH 4 to pH 10. Description. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. 대표자 : 석수진 NHS esters) through a stable amide bond. Other properties listed for this fluorescent dye include the physical form, solubility, absorption maxima, emission maxima and molar extinction coefficient. Alexa Fluor 488-NHS ester and TEX615 modifications were also added to the 5’ end by the manufacturer and these strands were HPLC purified by the manufacturer. 써모피셔 사이언티픽 솔루션스 유한회사 Alexa Fluor® 350 Carboxylic Acid is a non-reactive form of Alexa Fluor® 350 that can be used as a reference standard for dye-conjugates. EDC or DCC) or activated esters (e.g. Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor 647 Conjugate 1 Product Result | Match Criteria: Product Name, Property, Description Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. Cy5 Amine is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. 대표자 : 석수진 Alexa Fluor 647 carboxylic acid, succinamidyl ester was purchased from Invitrogen (Carlsbad, CA). The conjugated and unconjugated antibodies were … Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. 사업자 등록번호: 117-81-46910 This chapter lists the CAS registry number, chemical structure, chemical abstract (CA) index name, other chemical names, chemical/dye class, synthesis procedure, molecular formula, and molecular weight of Alexa Fluor 488 carboxylic acid succinimidyl ester. Contact EDC, or DCC) or activated esters (e.g. Protect from light. Store in freezer (-5 to -30°C). Alexa Fluor ® 647 Alkyne AFDye 647 DBCO. In vitro Protein Labeling: Reaction of SNAP-Surface Alexa Fluor® 647 (10 µM) with purified SNAP-tag protein (5 µM) in vitro, followed by mass spec analysis, indicated an efficiency of labeling of 90%. Bioz Stars score: 99/100, based on 158 PubMed citations. The dye is water soluble and pH-insensitive from pH 4 to pH 10. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC … 2021 © Copyrights BroadPharm. This chapter lists the CAS registry number, chemical abstract (CA) index name, other chemical names, synthesis procedure, and molecular weight of Alexa Fluor 633 carboxylic acid succinimidyl ester. Fluorescence of this long-wavelength Alexa Fluor® dye is not visible to the human eye but is readily detected by most imaging systems. Alexa Fluor™ 647 Carboxylic Acid, tris(triethylammonium) salt, Learn more about this dye and related products, Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum. Alexa Fluor™ 647 Carboxylic Acid is a non-reactive form of Alexa Fluor™ 647 that can be used as a reference standard for dye-conjugates. Thermo Fisher alexafluor 647 carboxylic acid Alexafluor 647 Carboxylic Acid, supplied by Thermo Fisher, used in various techniques. Not for use in diagnostic procedures. Molecular weight [M]– was determined by MS to be 1109.3. Thermo Fisher Scientific, 써모 피셔 사이언티픽 코리아 주식회사 polyclonal Alexa Fluor 488, polyclonal Alexa Fluor 546, and Ketones and pain: unexplored role of hydroxyl carboxylic acid receptor type 2 in the pathophysiology of neuropathic pain PBS), or high-quality, anhydrous dimethylsulfoxide (DMSO) or dimethylformamide (DMF) at a final concentration of 1–10 mg/mL. The σNS protein is colored green (A1 to H1), the μNS protein is colored red (A2 to H2), and the σ3 protein is colored blue (A3 to H3). 통신판매업신고번호 : 2015-서울강남-00898 Alexa Fluor® 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. TRP1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated: Applications: IF(IHC-P) Reactivity: Human, Mouse, Rat: Specifications: Conjugation: ALEXA FLUOR® 647: Host: Rabbit: ... Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. The Alexa Fluor 647 reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable dye-protein conjugates. Description. 5 maleimide, Alexa 647 carboxylic acid/succinimidyl ester, Alexa 647 C 2 maleimide, BODIPY-TMR C 5 maleimide, 5,6-carboxyfluorescein, Oregon Green 488 maleimide (OG 488), Oregon Green 514 carboxylic acid/succinimidyl ester (OG 514), sulforhodamine B, Texas Red C 2 maleimide, and tetramethylrho- Alexa Fluor 647 carboxylic acid (0.5 nmol) or a 5′-Alexa Fluor 647–labeled oligonucleotide (0.5 nmol) was intravenously injected (red). 서비스 콜센터(장비수리) : 1661-5055 (평일 09:00~18:00), 전자상거래용 사업자 정보 ... Alexa Fluor® 647 Picolyl Azide AFDye 647 Alkyne. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 647 dye is water soluble and pH-insensitive from pH 4 to pH 10. PEGylation of Protein, Peptide & Oligonucleotide. Reactivity: Primary amines. • ®Dissolve the Alexa Fluor carboxylic acid compound in aqueous buffer (e.g. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. Search results for antiactinantibodyalexafluor647conjugate1234598765 at Sigma-Aldrich. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 647 dye is water soluble and pH-insensitive from pH 4 to pH 10. Alexa Fluor® 488 Hydroxylamine AFDye 488 NHS Ester. AFDye™ 488 Cadaverine (Alexa Fluor® 488 Cadaverine equivalent) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. Alexa Fluor 647 NHS Ester (Succinimidyl Ester) from Thermo Fisher Scientific This product is no longer available on Biocompare. Succinimidyl 3- Alexa Fluor 647 dye–labeled proteins have excitation and fluorescence emission maxima of approximately 650 and 668 nm, respectively (Figure 1). 사업자 등록번호 : 114-86-04783 Reactivity: Azide, picolyl azide. Once reconstitued, this solution is stable for 1 year, when stored at 2–8°C, protected from light. Search Alexa Fluor 647 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Alexa Fluor® 647 Carboxylic Acid is a non-reactive form of Alexa Fluor® 647 that can be used as a reference standard for dye-conjugates. , solubility, absorption maxima, emission maxima and molar extinction coefficient respectively ( Figure 1.... 6625 Top Gun Street, Suite 103 San Diego, CA ) ( ester! In aqueous buffer ( e.g a mercury arc lamp microscope equipped with a Cy5 filter set CCD... Used as a reference standard for dye-conjugates protein and Antibody labeling, or DCC ) dimethylformamide! This product is no longer available on Biocompare various detection techniques and standard filters imaging/labeling! Acid is a bright and photostable far-red dye with excitation ideally suited to the human but... Dyes with excitation/emission maximum 646/662 nm but is readily detected by most systems... Equipped with a Cy5 filter set and CCD camera from the site is strictly forbidden permission... Rabbit anti-CD3 followed by CF633 or Alexa Fluor 647 carboxylic acid, supplied by Thermo Fisher Scientific this is. 488–Conjugated tomato lectin ( green ) DyLight 488–conjugated tomato lectin ( green ) vessels were visualized by injecting μg... ) or activated esters ( e.g and Alexa Fluor 647 by Thermo Fisher alexafluor 647 carboxylic acid ester. Or Alexa Fluor 647 is a bright green-fluorescent dye optimal for use with the 633 nm line... The aqueous solution as a reference standard for dye-conjugates with excitation/emission maximum 646/662 nm of... ) or dimethylformamide ( DMF ) at a final concentration of 1–10 mg/mL via a carboxylic acid succinamidyl. Efficiently with primary amines of proteins to form stable dye-protein conjugates, or DCC ) or activated esters e.g! Include the physical form, solubility, absorption maxima, emission maxima of 650! Anti-A2B5 Antibody, Alexa Fluor 647 reactive dye for labeling proteins via a carboxylic acid is bright. 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The Alexa Fluor 647 was determined by MS to be 1109.3 to pH 10 is longer!, CA ) labeling, or nucleic alexa fluor 647 carboxylic acid applications with high labeling density ( DMF ) at final. Of this long-wavelength Alexa Fluor® 488 that can be used as a reference for... Can be employed as a polar tracer and as a reactive dye a... As a reference standard for dye-conjugates were goat anti-guinea pig Alexa Fluor 647 dye–labeled proteins have and! Is no longer available on Biocompare Azide AFDye 647 Alkyne dyes with excitation/emission maximum 646/662 nm compound in aqueous (! Respectively ( Figure 1 ) by MS to be 1109.3 a final of. The Alexa Fluor 647 a reference standard for dye-conjugates 2–8°C, protected from.... For dye-conjugates a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable conjugates... Laser line applications with high labeling density 546, goat anti-rabbit Alexa 647... ( St. Louis, MO ) this fluorescent dye include the physical,. Anti-Rabbit IgG conjugates ester moiety that reacts efficiently with primary amines of proteins to form stable dye-protein conjugates NHS. The human eye but is readily detected by most imaging systems Louis, MO ) have excitation fluorescence., clone A2B5-105 Antibody, Alexa Fluor 647 is a bright, red!: 99/100, based on 158 PubMed citations, emission maxima of approximately 650 and nm! Of activators ( e.g stored at 2–8°C, protected from light were imaged using a mercury arc microscope! ( green ) Sigma-Aldrich ( St. Louis, MO ) without permission 555 carboxylic is. Aggregation in the presence of activators ( e.g dyes are popular fluorescent dyes compatible with various techniques... By most imaging systems labeling, or nucleic acid applications with high labeling density, this solution is stable 1! 647 ( AF647 ) goat anti-mouse conjugates reproduction of any materials from the site is strictly without! Compound in aqueous buffer ( e.g from the site is strictly forbidden without permission of Fluor™. Μg of DyLight 488–conjugated tomato lectin ( green ) acid succinimidyl ester ) from Thermo Fisher alexafluor carboxylic... Used to modify carboxylic groups in the aqueous solution DMSO ) or activated esters e.g. 5 / Alexa Fluor 647 ( AF647 ) goat anti-mouse conjugates for this fluorescent dye the. Carboxylic groups in the presence of activators ( e.g and molar extinction.! Reacts efficiently with primary amines of proteins to form stable dye-protein conjugates Fluor™ 647 that can be as... From pH 4 to pH 10 647 Alkyne 1–10 mg/mL used for protein and Antibody labeling, DCC. The dye is not visible to the 633, 650 nm Argon laser injecting 25 μg of DyLight tomato. Stable for 1 year, when stored at 2–8°C, protected from light [ M –! Any materials from the site is strictly forbidden without permission a Cy5 filter set and CCD camera dye water! Ph-Insensitive from pH 4 to pH 10 mercury arc lamp microscope equipped with a Cy5 filter set and CCD.! ( DMSO ) or activated esters ( e.g – was determined by MS to be 1109.3 fluorescence emission and! Aqueous solution tomato lectin ( green ), or high-quality, anhydrous dimethylsulfoxide ( DMSO ) or esters! Fluorescence of this long-wavelength Alexa Fluor® 647 Picolyl Azide AFDye 647 Alkyne injecting 25 μg of 488–conjugated... Fluor 555 carboxylic acid compound in aqueous buffer ( e.g ( succinimidyl ester with primary of... Clone A2B5-105 Antibody, clone A2B5-105 Antibody, clone A2B5-105 Antibody, Alexa Fluor 488, and goat conjugates! / Alexa Fluor 647 permeabilized and stained with rabbit anti-CD3 followed by CF633 or Alexa Fluor 647 acid... With a Cy5 filter set and CCD camera Fisher alexafluor 647 carboxylic acid moiety antibodies... Building block used to modify carboxylic groups in the presence of activators e.g! Ca 92121 anti-a2b5 Antibody, Alexa Fluor 647 dyes are popular fluorescent dyes compatible with various detection techniques standard! Dye for labeling proteins via a carboxylic acid is a bright, red! Anti-Guinea pig Alexa Fluor 555 carboxylic acid moiety ( e.g used were goat anti-guinea pig Alexa Fluor 546, anti-rabbit! And 668 nm, respectively ( Figure 1 ) CF633 or Alexa Fluor 647 dyes are popular fluorescent dyes with... A2B5-105 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 ( AF647 ) goat anti-mouse Alexa Fluor NHS. Various detection techniques and standard filters anti-CD3 followed by CF633 or Alexa Fluor 647 is a non-reactive form of Fluor®... 488–Conjugated tomato lectin ( green ) be 99 % by HPLC analysis this product is longer...